Materials
MATERIALS FOR BUILDING EACH OF THE THREE PHOTOAUTOTROPHIC VESSELS
* 1 - 2.54cm x 20.3cm diameter grey PVC (EV008)
* 1 – 2.54cm X 20.3cm diameter grey PVC (EV009)
* 1 - 1.9cm x 1.9cm grey PVC (EV010)
* 2 - 7.6cm diameter rubber gasket
* 2 - 2.54cm x 13.3cm diameter grey PVC (EV002)
* 1 – 1.27cm tee
* 1 - 3.8cm diameter x 10.2cm PVC rod (EV003)
* 2 – 1.9cm hose clamps
* 1 - 3.8cm diameter x 7.6cm PVC rod (EV004)
* 2 – icemaker tee values
* PVC glue
* 8 - ¼ - 20 unc x ¾ long hex head bolt
* Teflon tape
* 2 - PVC 1.27cm x 3 cm (airstone)
* 1 - #25 sprocket 24 tooth (mechanical agitation)
* 2 - .9525 dia. X 45.72 clear PVC hose
* 2 – air hydraulic rotary union (B-7248)
* 2 cubic feet of Helium
* 2 cubic feet of Neon
MATERIALS FOR BUILDING THE PHOTOAUTOTROPHIC BIOREACTOR
* 1 - Actron Vacuum Pump
* 2 – 1.3cm x 71cm x 40.6cm particle board
* 1 - #25 sprocket 12 tooth
* 3 – 30cm #25 metal chains
* 3 - #25 masterlink
* 3 – 1.25 hp gear motor 120 volt
* 2 – 2.5 cm x 2.5 cm x 63.5 aluminum angle
* screws, hand drill, drill bits, and screwdrivers
* 3 – on/off dimmer switches
MATERIALS FOR BUILDING THE NUTRIENT CONTROL CHAMBER (NCC)
* 1 - CO2-200 Meter by Amprobe
* 2 - ⅝ steel washers
* 2 - 1.2cm x 91cm x 61cm particle board
* 2 - 1.2cm x 66cm x 61cm particle board
* 2 – .6cm x 91cm x 61cm clear acrylic board
* 1 - .95cm valve
* 1 - 1.27cm x 3.8cm x 30.5cm pine board
* 1 - .95cm threaded union
* 2 - double outlet aquarium air pumps
* 1 - 7.6cm indoor mounting tape
* 2 - 1.27cm CPVC cap
* 2 - 183cm x 1.27cm x .95cm clear vinyl tube
* 1 - 1.27cm CPVC tee
* 1 - single electrical box
* 1 - 1.27cm x 12.7cm CPVC pipe
* 1 - nylon wallplate
* 2 - 1.27cm x ½ NPT reducing bushing
* 1 - 1.27cm CPVC union
MATERIALS FOR BUILDING THE LIPID EXTRACTOR/ TRANSESTERIFICATION CHAMBER (“CELLOUSE BLASTER”)
* 1 – EV A004 “cellulose blaster” assembly using cold roll steel
* 1 - Ultrasonic Frequency Generator made by Max Conditioning Technology
* 1- 200 psi pressure gage
* 1 - high pressure canister bottle
* 1 - ½” tee
* 2 –½” closed nipple
* 1 – ½” x 7.6 cm nipple
* 1 – ½” gas manual value
* 1 – ½” to ¼” reducing bushing
* 1 – ½” street elbow
* 1 – 1.3 cm high pressure PVC hose
* 6 – 1.3 cm hose clamps
* 2 - #8-32 x ½ brass screws
* 1 - Actron Vacuum Pump
MATERIALS FOR CULTIVATING ALGAE
* 500ml live Spirogyra culture for each vessel (x3)
* 1g of Iron Powder -100/+325 Mesh for each vessel (x3)
* 50cfm of Rhino Power Carbon Dioxide per vessel (x3)
* 1.5L of distilled water per vessel (x3)
* Helium
* Neon
* 23g Sodium Carbonate per vessel (x3)
MATERIALS FOR QUANTIFYING ALAGE GROWTH
* pH meter
* Hemacytometer
* sanitizing spray
* 60ml syringe
* fire extinguisher
* Microscope, magnification 40x-400x
* lab coat
* 8 microscope slides
* ventilation mask
* 3 small test tubes
* disposable gloves
* Pipettes
* apron
* Safety Equipment
* goggles
MATERIALS FOR HARVESTING ALGAE BIOMASS
* Iron Oxide
* 7.6 cm diameter N45 Neodymium Magnet
* ventilation mask
* 6 large test tubes
* disposable glove
* Pipettes
* apron
* Safety Equipment
* goggles
* gram scale
MATERIALS FOR LIPID EXTRACTION
* Cellulose Blaster
* Sodium Chloride
* 2 - Erlenmeyer Beakers
* ventilated hood
* googles, labcoat, gloves, ventilated mask
* autoclave
* gram scale
MATERIALS FOR TRANSESTERIFICATION
* Cellulose Blaster
* Methanol
* Barium Hydroxide
* 2 - Erlenmeyer Beakers
* Distilled water
* ventilated hood
* googles, labcoat, gloves, ventilated mask
* autoclave
* gram scale
The following apparatuses were built for starting the algae cultures:
MATERIALS FOR BUILDING LIGHT SPECTRUM PHOTO-BIOREACTOR
* 3 – 15 watt florescent grow light fixtures
* 118cm of adhesive Velcro
* 2- 2030cm round particle boards
* 4- 1.27cm x 5.1cm x 45.7cm plastic supports
* One roll of silver duct tape
* 2 windshield reflector screens cut to size
* 16 drywall screws
* light timer
* Gorilla glue
* Electric hand saw
* Jigsaw
* Krylon spray paint
* Hand drill, drill bits, and screwdrivers
MATERIALS FOR BUILDING INCUBATORS
* 4 double outlet aquarium air pumps
* 1 digital thermometer
* 2 air spargers
* 8 – coffee stirrers
* 2 – 8.89cm rubber collars
* 8 – 15.24cm of 3.175mm clear flex tubing
* 2 – inner tube value stem
* 4 – icemaker tee values
* 2 – 43.18cm of 7.62cm diameter clear PVC pipe
* 2 - 3.81cm cross fitting
* 2 - 2.69cm x 2.54cm diameter white PVC pipe
* 4 - 3.81cm to 2.54cm reducing bushings
* 2 - 3.17cm of 1.27cm diameter white PVC pipe
* 2 – 7.62cm to 3.81cm reducing bushings
* 2 - 3.49cm of 3.8cm diameter white PVC pipe
* 2 - 3.81cm to 1.27cm reducing bushings
* 2 – 3.02cm of 1.27cm diameter white PVC pipe
* 2 – 7.62cm caps
* 2 – 8.57cm of 1.27cm diameter white PVC pipe
* 2 – 1.27cm slip by thread bushing
* 2 – 22.7cm of 1.27cm diameter white PVC pipe
* 2 – 1.27cm hose bibs
* 2 – 5.08 cm x 62cm diameter white PVC pipe
* 2 – 1.27cm caps
* 2 – 2.54cm x 3.81cm diameter white PVC pipe
* 2 – 1.27cm elbow
* 1 – 0-100 PSI gage
* Hand drill, miter saw, PVC glue, Teflon Tape
* 1 - 2.54cm x 20.3cm diameter grey PVC (EV008)
* 1 – 2.54cm X 20.3cm diameter grey PVC (EV009)
* 1 - 1.9cm x 1.9cm grey PVC (EV010)
* 2 - 7.6cm diameter rubber gasket
* 2 - 2.54cm x 13.3cm diameter grey PVC (EV002)
* 1 – 1.27cm tee
* 1 - 3.8cm diameter x 10.2cm PVC rod (EV003)
* 2 – 1.9cm hose clamps
* 1 - 3.8cm diameter x 7.6cm PVC rod (EV004)
* 2 – icemaker tee values
* PVC glue
* 8 - ¼ - 20 unc x ¾ long hex head bolt
* Teflon tape
* 2 - PVC 1.27cm x 3 cm (airstone)
* 1 - #25 sprocket 24 tooth (mechanical agitation)
* 2 - .9525 dia. X 45.72 clear PVC hose
* 2 – air hydraulic rotary union (B-7248)
* 2 cubic feet of Helium
* 2 cubic feet of Neon
MATERIALS FOR BUILDING THE PHOTOAUTOTROPHIC BIOREACTOR
* 1 - Actron Vacuum Pump
* 2 – 1.3cm x 71cm x 40.6cm particle board
* 1 - #25 sprocket 12 tooth
* 3 – 30cm #25 metal chains
* 3 - #25 masterlink
* 3 – 1.25 hp gear motor 120 volt
* 2 – 2.5 cm x 2.5 cm x 63.5 aluminum angle
* screws, hand drill, drill bits, and screwdrivers
* 3 – on/off dimmer switches
MATERIALS FOR BUILDING THE NUTRIENT CONTROL CHAMBER (NCC)
* 1 - CO2-200 Meter by Amprobe
* 2 - ⅝ steel washers
* 2 - 1.2cm x 91cm x 61cm particle board
* 2 - 1.2cm x 66cm x 61cm particle board
* 2 – .6cm x 91cm x 61cm clear acrylic board
* 1 - .95cm valve
* 1 - 1.27cm x 3.8cm x 30.5cm pine board
* 1 - .95cm threaded union
* 2 - double outlet aquarium air pumps
* 1 - 7.6cm indoor mounting tape
* 2 - 1.27cm CPVC cap
* 2 - 183cm x 1.27cm x .95cm clear vinyl tube
* 1 - 1.27cm CPVC tee
* 1 - single electrical box
* 1 - 1.27cm x 12.7cm CPVC pipe
* 1 - nylon wallplate
* 2 - 1.27cm x ½ NPT reducing bushing
* 1 - 1.27cm CPVC union
MATERIALS FOR BUILDING THE LIPID EXTRACTOR/ TRANSESTERIFICATION CHAMBER (“CELLOUSE BLASTER”)
* 1 – EV A004 “cellulose blaster” assembly using cold roll steel
* 1 - Ultrasonic Frequency Generator made by Max Conditioning Technology
* 1- 200 psi pressure gage
* 1 - high pressure canister bottle
* 1 - ½” tee
* 2 –½” closed nipple
* 1 – ½” x 7.6 cm nipple
* 1 – ½” gas manual value
* 1 – ½” to ¼” reducing bushing
* 1 – ½” street elbow
* 1 – 1.3 cm high pressure PVC hose
* 6 – 1.3 cm hose clamps
* 2 - #8-32 x ½ brass screws
* 1 - Actron Vacuum Pump
MATERIALS FOR CULTIVATING ALGAE
* 500ml live Spirogyra culture for each vessel (x3)
* 1g of Iron Powder -100/+325 Mesh for each vessel (x3)
* 50cfm of Rhino Power Carbon Dioxide per vessel (x3)
* 1.5L of distilled water per vessel (x3)
* Helium
* Neon
* 23g Sodium Carbonate per vessel (x3)
MATERIALS FOR QUANTIFYING ALAGE GROWTH
* pH meter
* Hemacytometer
* sanitizing spray
* 60ml syringe
* fire extinguisher
* Microscope, magnification 40x-400x
* lab coat
* 8 microscope slides
* ventilation mask
* 3 small test tubes
* disposable gloves
* Pipettes
* apron
* Safety Equipment
* goggles
MATERIALS FOR HARVESTING ALGAE BIOMASS
* Iron Oxide
* 7.6 cm diameter N45 Neodymium Magnet
* ventilation mask
* 6 large test tubes
* disposable glove
* Pipettes
* apron
* Safety Equipment
* goggles
* gram scale
MATERIALS FOR LIPID EXTRACTION
* Cellulose Blaster
* Sodium Chloride
* 2 - Erlenmeyer Beakers
* ventilated hood
* googles, labcoat, gloves, ventilated mask
* autoclave
* gram scale
MATERIALS FOR TRANSESTERIFICATION
* Cellulose Blaster
* Methanol
* Barium Hydroxide
* 2 - Erlenmeyer Beakers
* Distilled water
* ventilated hood
* googles, labcoat, gloves, ventilated mask
* autoclave
* gram scale
The following apparatuses were built for starting the algae cultures:
MATERIALS FOR BUILDING LIGHT SPECTRUM PHOTO-BIOREACTOR
* 3 – 15 watt florescent grow light fixtures
* 118cm of adhesive Velcro
* 2- 2030cm round particle boards
* 4- 1.27cm x 5.1cm x 45.7cm plastic supports
* One roll of silver duct tape
* 2 windshield reflector screens cut to size
* 16 drywall screws
* light timer
* Gorilla glue
* Electric hand saw
* Jigsaw
* Krylon spray paint
* Hand drill, drill bits, and screwdrivers
MATERIALS FOR BUILDING INCUBATORS
* 4 double outlet aquarium air pumps
* 1 digital thermometer
* 2 air spargers
* 8 – coffee stirrers
* 2 – 8.89cm rubber collars
* 8 – 15.24cm of 3.175mm clear flex tubing
* 2 – inner tube value stem
* 4 – icemaker tee values
* 2 – 43.18cm of 7.62cm diameter clear PVC pipe
* 2 - 3.81cm cross fitting
* 2 - 2.69cm x 2.54cm diameter white PVC pipe
* 4 - 3.81cm to 2.54cm reducing bushings
* 2 - 3.17cm of 1.27cm diameter white PVC pipe
* 2 – 7.62cm to 3.81cm reducing bushings
* 2 - 3.49cm of 3.8cm diameter white PVC pipe
* 2 - 3.81cm to 1.27cm reducing bushings
* 2 – 3.02cm of 1.27cm diameter white PVC pipe
* 2 – 7.62cm caps
* 2 – 8.57cm of 1.27cm diameter white PVC pipe
* 2 – 1.27cm slip by thread bushing
* 2 – 22.7cm of 1.27cm diameter white PVC pipe
* 2 – 1.27cm hose bibs
* 2 – 5.08 cm x 62cm diameter white PVC pipe
* 2 – 1.27cm caps
* 2 – 2.54cm x 3.81cm diameter white PVC pipe
* 2 – 1.27cm elbow
* 1 – 0-100 PSI gage
* Hand drill, miter saw, PVC glue, Teflon Tape